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Journal Articles


57. Bundy, B. C., Hunt, J. P., Jewett, M. C., Swartz, J. R., Wood, D. W., Frey, D. D., Rao, G., “Cell-free biomanufacturing,” Current Opinion in Chemical Engineering, Vol. 22, pp. 177-183, (2018).

56. Fan, Y., Miozzi, J. M., Stimple, S. D., Han, T-C. and Wood, D. W., “Column-Free Purification Methods for Recombinant Proteins Using Self-Cleaving Aggregating Tags,” Polymers, Vol. 10 (5), p. 468, (2018).

55. Gurramkonda, C., Rao, A,. Borhani, S., Pilli, M., Deldari, S., Ge, X., Pezeshk, N., Han, T-C, Tolosa, M., Kostov, Y., Tolosa, L., Wood, D. W., Vattem, K., Frey, D. D., Rao, G., “Improving the recombinant human erythropoietin glycosylation using microsome supplementation in CHO cell-free system,” Biotechnology and Bioengineering, Vol. 115 (5), pp. 1253-1264, (2018).

54. Tran, K., Gurramkonda, C., Cooper, M. A., Pilli, M., Taris, J. E., Selock, N., Han, T-C., Tolosa, M., Zuber, A., Peñalber-Johnstone, C., Dinkins, C., Pezeshk, N., Kostov, Y., Frey, D. D., Tolosa, L., Wood, D. W., and Rao, G., “Cell-free production of a therapeutic protein: Expression, purification, and characterization of recombinant streptokinase using a CHO lysate,” Biotechnology and Bioengineering, Vol. 115 (1), pp. 92-102, (2018).

53. Cooper, M. A.,* Taris, J. E.,* Shi, C. and Wood, D. W., “A convenient self-cleaving affinity tag method for the purification of tagless target proteins (Invited Methods Paper),” Current Protocols in Protein Science, Vol. 91, pp. 5.29.1-5.29.23, (2018).

52. Lahiry, A., Fan, Y., Stimple, S. E., Raith, M. and Wood, D. W., “Inteins as tools for tagless and traceless protein purification,” Journal of Chemical Technology and Biotechnology, Vol. 93, pp. 1827-1835, (2018).


51. Salehi, A. S. M., Shakalli Tang, M. J., Smith, M. T., Hunt, J. M., Law, R. A., Wood, D. W. and Bundy, B. C., “Cell-Free Protein Synthesis Approach to Biosensing hTRβ-Specific Endocrine Disruptors,” ACS Analytical Chemistry, Vol. 89 (6), pp. 3395-3401, (2017).

50. Lahiry, A., Stimple, S. D., Wood, D. W., Lease, R., “Retargeting a dual-acting sRNA for multiple mRNA transcript regulation,” ACS Synthetic Biology, Vol. 6 (4), pp. 648-658, (2017).

49. Coolbaugh, M. J., Shakalli Tang, M. J., Wood, D. W., “High-throughput purification of recombinant proteins using self-cleaving intein tags,” Analytical Biochemistry, Vol. 516, pp. 65-74, (2017).


48. Cronin, M., Coolbaugh, M. J., Nellis, D., Zhu, J., Wood, D. W., Nussinov, R. & Ma, B., “Dynamics differentiate between active and inactive inteins,” European Journal of Medicinal Chemistry, Vol. 91, pp. 51-62, (2015).


47. Shi, C., Tarimala, A., Qing Meng, Q. & Wood, D. W., “A general purification platform for toxic proteins based on intein trans-splicing,” Applied Microbiology and Biotechnology, Vol. 98 (22), pp. 9425-35, (2014).

46. Wood, D. W., “New Trends and Affinity Tag Designs for Recombinant Protein Purification,” Current Opinion in Structural Biology, Vol. 26, pp. 54-61, (2014).

45. Wood, D. W. & Camarero, J. A., “Intein Applications: From Protein Purification and Labeling to Metabolic Control Methods,” Journal of Biological Chemistry, Vol. 289 (21), pp. 14512–14519, (2014).

44. Shi, C., Qing Meng, Q. & Wood, D. W., “A peptide-triggered intein-based self-cleaving non-chromatographic purification tag for recombinant protein purification,” Chinese Journal of Biochemistry and Molecular Biology, Vol. 30 (2), pp. 202-208, (2014).

43. Shi, C., Qing Meng, Q. & Wood, D. W., “Analysis of the roles of mutations in thyroid hormone receptor- β by a bacterial biosensor system,” Journal of Molecular Endocrinology, Vol. 52 (1), pp. 55–66, (2014)


42. Shi, C., Miskioglu. E. E., Qing Meng, Q. & Wood, D. W., “Intein-based purification tags in recombinant protein production, and new methods for controlling self-cleavage,” Pharmaceutical Bioprocessing, Vol. 1 (5), pp. 441–454, (2013).

41. Warren, T. D., Coolbaugh, M. J. & Wood, D. W., “Ligation-independent cloning and self-cleaving intein as a tool for high-throughput protein purification,” Protein Expression and Purification, Vol. 91, pp. 169-174, (2013).

40. Shi, C., Qing Meng, Q. & Wood, D. W., “A dual ELP tagged split intein system for non-chromatographic recombinant protein purification,” Applied Microbiology and Biotechnology, Vol. 97 (2), pp. 829-35, (2013).


39. Gierach, I., Li, J., Wu, W.-Y. & Wood, D. W, "Engineered Human Thyroid Receptor α-1 and β-1 Biosensors for Screening TR Subtype-Selective Ligands." FEBS Open Bio, 2 247-253

38. Li, J., Gierach, I., Gillies, A. R.,Warden, C. D. & Wood, D. W., "Engineering and Optimization of an Allosteric Peroxisome Proliferator-Activated Receptor Gamma Protein Biosensor." Biosensors and Bioelectronics.


37. Li, J., Gierach, I., Gillies, A. R.,Warden, C. D. & Wood, D. W., “Engineering and Optimization of an Allosteric Peroxisome Proliferator-Activated Receptor Gamma Protein Biosensor,” Biosensors and Bioelectronics, Vol. 29, pp. 132-139, (2011).

36. Gierach, I., Shapero, K., Eyster, T. W. & Wood, D. W. “Bacterial Biosensors for Evaluating Potential Impacts of Estrogenic Endocrine Disrupting Compounds in Multiple Species,” Environmental Toxicology, Vol. 26 (3), pp. 1-11, (2011).

35. Wu, W.-Y., Miller, K. D., Coolbaugh, M. J. & Wood, D. W., “Intein-mediated One-step Purification of E. coli Secreted Human Antibody Fragments,” Protein Expression and Purification, Vol. 76, pp. 221-228, (2011).


34. Fong, B. A. & Wood, D. W., “Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation,” Microbial Cell Factories, Vol. 9, p. 77, (2010).

33. Wood, D. W., “Non-chromatographic Recombinant Protein Purification by Self-Cleaving Purification Tags,” Separations Science and Technology, Vol. 45 (15), pp. 2345-2357, (2010).

32. Fong, B. A., Gillies, A. R., Ghazi, I., LeRoy, G., Lee, K. C., Westblade, L. F. & Wood, D. W., “Purification of Escherichia coli RNA polymerase using a self-cleaving elastin-like polypeptide tag,” Protein Science, Vol. 19 (6), pp. 1243-1252, (2010).

31. Wu, W. Y., Gilles, A. R., Hsii, J., Contreras, L., Oak, S., Perl, M. B., & Wood, D. W., “Self-cleaving purification tags re-engineered for rapid Topo® cloning,” Biotechnology Progress, Vol. 26 (5), pp. 1205-1212, (2010).

30. Fong, B. A., Wu, W.-Y. & Wood, D. W., “The potential role of self-cleaving purification tags in commercial-scale processes,” Trends in Biotechnology, Vol. 28 (5), pp. 272-279, (2010).


29. Fong,Baley,A; Wu,Wan-Yi; Wood,David,W, "Optimization of ELP-intein mediated protein purification by salt substitution." Protein Expression and Purification, 66 2 198-202

28. Hartman, I., Gillies, A. R., Arora, S., Andaya, C., Royapet, N., Welsh, W. J., Zauhar, R J. & Wood, D. W.,,"Novel Screening Methods Using Shape Signatures and Engineered Biosensors for Identification of Estrogen Antagonists." Pharmaceutical Research, 26 10 2247-2258

27. Hartman,Izabela; Gillies,Alison,R; Arora,Sonia; Andaya,Christina; Royapet,Nitya; Welsh,William,J; Wood,David,W; Zauhar,Randy,J, "Application of Screening Methods, Shape Signatures and Engineered Biosensors in Early Drug Discovery Process." Pharmaceutical Research, 26 10 2247-2258

26. Wu, W.-Y., Fong, B. A., Gillies, A. R. & Wood, D. W., "Recombinant Protein Purification by Self-cleaving Elastin-like Polypeptide Fusion Tag." Current Protocols in Protein Science, 26 4 1-18

25. Gawrys,Michelle,D; Hartman,Izabela; Landweber,Laura,F; Wood,David,W, "Use of engineered Escherichia coli Cells to Detect Estrogenicity in Everyday Consumer Products." Journal of Chemical Technology and Biotechnology, 84 12 1834-1840

24. Fong, B. A., Wu, W.-Y. & Wood, D. W., “Optimization of ELP-intein mediated protein purification by salt substitution,” Protein Expression and Purification, Vol. 66 (2), pp. 198-202, (2009).


23. Gillies, A. R., Hsii, J. F., Oak, S. & Wood, D. W., “Rapid cloning and purification of proteins: Gateway vectors for protein purification by self-cleaving tags (Editors’ Choice feature publication),” Biotechnology and Bioengineering, Vol. 101 (2), pp. 229-240, (2008).

22. Staii, C., Wood, D. W. & Scoles, G., “Ligand-induced Structural Changes in Maltose Binding Proteins Measured by Atomic Force Microscopy,” Nano Letters, Vol. 8 (8), pp. 2503-2509, (2008).

21. Staii, C., Wood, D. W. & Scoles, G., “Verification of biochemical activity for proteins nanografted on gold surfaces,” Journal of the American Chemical Society, Vol. 130 (2), pp. 640-646, (2008).

20. Gillies, A. R.; Skretas, G.; Wood, D. W., “Engineered Systems for Detection and Discovery of Nuclear Hormone-Like Compounds,” Biotechnology Progress, Vol. 24, pp. 8-16, (2008).

19. Mee, C., Banki, M. R. and Wood, D. W., “Towards the Elimination of Chromatography in Protein Purification: Expressing Proteins Engineered to Purify Themselves,” Chemical Engineering Journal, Vol. 135, pp. 56-62, (2008).


18. Skretas,Georgios; Meligova,Aggeliki,K; Villalonga-Barber,Carolina; Mitsiou,Dimitra,J; Alexis,Michael,N; Micha-Screttas,Maria; Steele,Barry,R; Screttas,Constantinos,G; Wood,David,W, "Engineered chimeric enzymes as tools for drug discovery: Generating reliable bacterial screens for the detection, discovery, and assessment of estrogen receptor modulators." Journal of the American Chemical Society, 129 27 8443-8457


17. Wu, W.-Y, Mee, C., Califano, F., Banki, R. & Wood, D.W., “Recombinant Protein Purification by Self-Cleaving Aggregation Tag,” Nature Protocols, Vol. 1, pp. 2257-2262, (2006).


16. Skretas, G. & Wood, D.W., “Rapid Detection of Subtype-Selective Nuclear Hormone Receptor Binding with Bacterial Genetic Selection,” Applied and Environmental Microbiology, Vol. 71, pp. 8995-8997, (2005).

15. Banki, M.R. & Wood, D.W., “Inteins and Affinity Resin Substitutes for Protein Purification and Scale Up,” Microbial Cell Factories, Vol. 4:32, (2005).

14. Banki, M.R., Feng, L. & Wood, D.W., “Simple Bioseparations Using Self-Cleaving Elastin-Like Polypeptide Tags,” Nature Methods, Vol. 2, pp. 659-661, (2005).

13. Barnard, G.C., McCool, J.D., Wood, D.W. & Gerngross T.U., “An Integrated Recombinant Protein Expression and Purification Platform,” Applied and Environmental Microbiology, Vol. 71, pp. 5735-5742 (2005).

12. Banki, M.R., Gerngross, T.U. & Wood, D.W., “Novel and Economical Purification of Recombinant Proteins: Intein-Mediated Protein Purification Using In Vivo Polyhydroxybutyrate (PHB) Matrix Association,” Protein Science, Vol. 14, pp. 1387-1395, (2005).

11. Bradley, L.H., Kleiner, R.E., Wang, A.F., Hecht, M.H. & Wood, D.W., “An Intein-Based Genetic Selection Allows the Construction of a High-Quality Library of Binary Patterned De Novo Protein Sequences,” Protein Engineering Design and Selection, Vol. 18, pp. 201-207, (2005).

10. Skretas, G. & Wood, D.W., “A Bacterial Biosensor of Endocrine Modulators,” Journal of Molecular Biology, Vol. 349, pp. 464-474, (2005).

9. Skretas, G. & Wood, D.W., “Regulation of Protein Activity with Small-Molecule-Controlled Inteins,” Protein Science, Vol. 14, pp. 523-532, (2005).


8. Wood, D., "Simplified Protein Purification Using Engineered Self-Cleaving Affinity Tags." Journal of Chemical Technology and Biotechnology, 78 103-110


7. Wu, W., Wood, D., Belfort, G., Derbyshire, V. & Belfort, M., "Intein-Mediated Purification of Cytotoxic Endonuclease I-TevI by Insertional Inactivation and pH-Controllable Splicing." Nucleic Acids Research, 30 224864-4871


6. Wood, D., "Conquering the Proteome: Complexity, Cooperation and Commerce (Invited Meeting Report)." Trends in Biotechnology, 19 375-376


5. Wood, D., Derbyshire, V., Wu, W., Chartrain, M., Belfort, M. and Belfort, G., "Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor." Biotechnology Progress, 16 6 1055-1063

4. Pieracci,J; Wood,D,W; Crivello,J,V; Belfort,G, "UV-Assisted Graft Polymerization of N-Vinyl-2-Pyrrolidinone onto Poly(Ether Sulfone) Ultrafiltration Membranes: Comparison of Dip Versus Immersion Modification Techniques." Chemistry of Materials, 12 8 2123-2133


3. Wood,D,W; Wu,W; Belfort,G; Derbyshire,V; Belfort,M, "A Genetic System Yields Self-Cleaving Inteins for Bioseparations." Nature Biotechnology, 17 9 889-892

2. Kluge,T; Rezende,C; Wood,D; Belfort,G, "Protein Transmission During Dean Vortex Microfiltration of Yeast Suspensions." Biotechnology and Bioengineering, 65 6 649-658


1. Derbyshire, V., Wood, D., Wu, W., Dansereau, J., Dalgaard, J. and Belfort, M., “Genetic Definition of a Protein-Splicing Domain: Functional Mini-Inteins Support Structure Predictions and a Model for Intein Evolution,” Proc. Natl. Acad. Sci. USA, Vol. 94, pp. 11466-11471, (1997).